2 edition of Gene expression in the human myometrium during pregnancy and labour found in the catalog.
Gene expression in the human myometrium during pregnancy and labour
Tiong Ghee Teoh
Written in English
Thesis (M.D.) - University College Dublin, National University of Ireland, 1996.
|Statement||Tiong Ghee Teoh.|
Explore the latest full-text research PDFs, articles, conference papers, preprints and more on HIGH THROUGHPUT SEQUENCING. Find methods information, sources, references or conduct a literature. Spatial and temporal expression of splicing factors in the myometrium during pregnancy.(A) SF2/ASF, hnRNPA1, SRp40, SC35, PSF and UI-A snRNP protein expression within the upper and lower uterine regions of the human are shown as the mean (n = 10) for non-pregnant (NP), term not in labour; upper (PU) and lower uterine regions and labouring upper (LU) and lower uterine .
Certain novel genes were found to be upregulated in human myometrium at labor. Of these, PSCDBP, TLR2, TWIST1, FLJ, andRGS12 have not been previously characterized or identified in human myometrium. EDNRB is the other novel labor-associated gene whose reported expression is also upregulated at labor. In addition, we compared the effects of lipoxin A(4) ( nM) with vehicle on basal and LPS-stimulated expression of proinflammatory cytokines from samples of myometrium from pregnant women. Mean ± SE circulating level of lipoxin A(4) was ± nM at wk gestation, with a further modest increase during pregnancy (P.
The 9-kilodalton vitamin D-dependent calcium-binding protein (CaBP9K) is expressed in the intestine and uterus of mammals. In this study, we demonstrated the intracellular location of CaBP9K and quantified its expression in human myometrial tissues from nonpregnant and pregnant women (before and after the onset of labor). human uterus, and validated them for the purpose of studying human pregnancy and labour. The specific objectives of this study were to monitor the viability and characterize the expression profile using selected cellular, contractile and pregnancy associated markers in the primary cultured human myometrial cells. Labour has been.
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The majority of the change in gene expression occurred in pregnant myometrium, and further studies of these novel genes may help to elucidate their roles in facilitating adaptations of the uterus to pregnancy, including the expansion of myometrium in the pregnant uterus, and the maintenance of uterine quiescence during the majority of by: Expression of myometrial CREM (28 kDa) and ATF2-like (28 kDa) proteins in the upper and lower uterine segments during pregnancy and labour.
From Western blot analysis the two main cAMP-dependent transcription factors that are expressed in the human myometrium during gestation and parturition appear to be the CREM 28 kDa and ATF2-like 28 kDa.
Our pilot data showing a significant MMP mRNA increase during labor thus parallels the reported protein expression and activity in the myometrium. Similarly, much data has been gathered on IL IL-8 IR has been previously identified in human amnion, amniotic fluid, chorion, decidua, and villous placenta, and its level increases during labor Cited by: Cell adhesion molecule expression (ICAM1) increases in the human myometrium during late pregnancy and parturition.
These changes can be attributed to leukocytes infiltrating these tissues [ 33 ]. We noted that the increased expression of ICAM at term was not restricted to ICAM1 but also involved by: We and others have shown that 20α-HSD expression is upregulated in the mouse and human myometrium prior to labour [73,], and is negatively regulated by a transcription factor STAT5b whose expression declines at term [,].
20α-HSD-deficient mice showed high systemic P4 levels at term and experienced delayed parturition, while Cited by: 1. The smooth muscle coat of the uterus, which forms the main mass of the organ. | Explore the latest full-text research PDFs, articles, conference papers, preprints and more on MYOMETRIUM.
Find. Here we study the origin of eutherian pregnancy by investigating gene expression in a key component of the mouse and human myometrium uterus and cervix during pregnancy and labor.
The hypothesis posits that for most of human pregnancy, when myometrial cells express mainly PR-B (i.e. PR-A to PR-B ratio gene expression and that functional progesterone withdrawal at parturition is mediated by increased expression of PR-A (i.e. PR-A to PR-B ratio >1. In the baboon, COX-2 expression was increased in the lower uterine segment, cervix, and decidua but not in the uterine fundus, chorion, and placenta during late pregnancy and labor.
In humans, a large increase in COX-2 mRNA was found throughout late gestation in fetal tissues [ 32 ]. We hypothesized that the expression of myometrial activation genes (OTR, FP, Cx) would increase at both preterm and term labor relative to gestational age-matched controls or earlier in gestation and that 2) myometrial stimulation genes and effectors (e.g.
PGHS-2 and prostaglandins) would increase at both term and preterm labor relative to gestational age-matched controls or earlier in gestation. Microarray analysis of uterine gene expression in mouse and human pregnancy. Mol Endocrinol. ; – Aguan K, Carvajal JA, Thompson LP, Weiner CP.
Application of a functional genomics approach to identify differentially expressed genes in human myometrium during pregnancy and labour. Mol Hum Reprod. ; – Here, we report expression of 17β-hydroxysteroid dehydrogenase (HSD) type 2 and 20α-HSDs in the human cervix.
Data are presented to support the hypothesis that during pregnancy, expression of 17βHSD type 2 prevents ripening of the cervix by its oxidative 20αHSD activity, thereby maintaining elevated intracervical progesterone levels. This study provides the first evidence for differential expression of key signalling components of the mTOR pathway in the human myometrium during pregnancy.
In addition, this study is the first to identify and validate the patterns of gene expression associated with myometrial mTOR signalling in P4-treated cells. We conclude that estrogenic actions in the human myometrium during pregnancy, including the stimulation of contraction-associated gene expression, can be mediated by extranuclear signaling through ERα via activation of the ERK/mitogen-activated protein kinase pathway.
The expression of MMP and TIMP-1 was determined in human term and preterm myometrium before the onset of labor (TNL, n = 7; PTNL, n = 7) and during active labor (TL, n = 8; PTL, n = 8). Gene expression and tissue localization were assessed by quantitative polymerase chain reaction and immunohistochemistry, respectively.
Curley and coworkers detected the mRNA expression of KirKirSUR1 and SUR2B in human pregnant myometrium and demonstrated that Kir/SUR2B may be the predominant isoform of KATP channel in human myometrium. However, no information is available on the protein expression of KATP channel subunits in myometrium.
We propose that the presence of H3K27ac and H3K4me3 marks at labor-associated gene promoters and putative intergenic enhancers in the precontractile mouse myometrium as early as gestational day 15 epigenetically activates these regulatory regions, even at a stage of pregnancy when the expression of many labor-associated genes is comparatively low.
Myometrium of women in spontaneous labor at term is characterized by a stereotypic gene expression pattern consistent with over-expression of the inflammatory response and leukocyte chemotaxis.
Differential gene expression identified with microarray was confirmed with qRT-PCR using an independent set of samples. It is to notice that expression of genes involved in growth and cytoskeletal remodeling of myometrial cells was critically modified in the comparison of the non pregnant versus pregnant uterus, until term.
Conversely, limited changes in gene transcription, characterized the transition between the term and term in labor stages in the myometrium.
During human labour, we have reported that there is an increase in the ratio of PRA/PRB and suggested that PRA acts to antagonize the repressive functions of PRB on labour gene expression 6.
Here. (). Expression of regulator of G protein signaling-2 in rat myometrium during pregnancy and parturition. (). Expression of secretory and cytosolic phospholipase A2 in human myometrium: Changes in relation to gestational age and labour.
(). Gene expression proﬁling of rat uterus at different stages of parturition. ().Explore the latest full-text research PDFs, articles, conference papers, preprints and more on ECM. Find methods information, sources, references or conduct a literature review on ECM.expression and regulation in human myometrium Evonne C.
Chin-Smith1, Frances R. Willey1, Donna M. Slater2, Michael J. Taggart3 and Rachel M. Tribe1* Abstract Background: During pregnancy, myometrial gene and protein expression is tightly regulated to accommodate fetal growth, promote quiescence and ultimately prepare for the onset of labour.